Spasmolytic and Antibacterial Activity of TwoCitrus sinensisOsbeck Varieties Cultivated in Mexico

Abstract

Antibacterial activity on ATCC strains ofEscherichia coli,Salmonella enterica,Salmonella enteritidis,andSalmonella choleraesuisand spasmolytic effect on contraction on rat ileum trips were determinate. Eight organic extracts (hexanic and methanolic) of albedo (mesocarp) and flavedo (pericarp) of two varieties (Valencian and National) ofCitrus sinensis(L.) Osbeck of Yucatán, México, were studied. Additionally, chromatographic fingerprints were obtained and correlated with their pharmacological effects. MAN, MAV, and HFN extract caused inhibition againstS. choleraesuis(MIC: 1000 µg/mL) andS. enteritidis(MIC: 1000µg/mL). Regarding the spasmolytic effect, the Valencian extracts variety was more efficient on spontaneous contraction, HAV (Emax=51.98±1.98%), MAV (Emax=35.98±1.42%), HFV (Emax=68.91±4.14%), and MFV (Emax=51.28±2.59%), versus National variety, HAN (Emax=43.80±6.32%), MAN (Emax=14.62±1.69%), HFN (Emax= 64.87 ± 3.04%), and MFN (Emax=31.01±3.92%). Chromatographic fingerprints of HFV and HFN were found to have some similar signals that belong to monoterpenes, whereas for HAN and HAV similar signals were found belonging to fatty acids and triterpenoids. Methanolic extracts showed signals of (1) furfural, (2) furfural acetone (3) furfuraldehyde and (4)β–sitosterol compounds. Flavedo portion ofC. sinensispossessed spasmolytic effect on rat ileum strips and antibacterial activity againstSalmonellastrains. This species is source for obtaining bioactive compounds with therapeutic potential in the treatment of infectious diarrhea.