Preparation of Biocompatible Carboxymethyl Chitosan Nanoparticles for Delivery of Antibiotic Drug

Author:

Zhao Liang1,Zhu Bingya1,Jia Yunhong1,Hou Wenjiu2,Su Chang2

Affiliation:

1. College of Pharmacy, Liaoning Medical University, Jinzhou 121000, China

2. College of Veterinary Medicine, Liaoning Medical University, Jinzhou 121000, China

Abstract

Objective. To prepare biocompatible ciprofloxacin-loaded carboxymethyl chitosan nanoparticles (CCC NPs) and evaluate their cell specificity as well as antibacterial activity againstEscherichia coliin vitro.Methods. CCC NPs were prepared by ionic cross-linking method and optimized by using Box-Behnken response surface method (BBRSM). Zeta potential, drug encapsulation, and release of the obtained nanoparticles in vitro were thoroughly investigated. Minimum inhibitory concentration (MIC) and killing profiles of free or ciprofloxacin-loaded nanoparticles againstEscherichia coliwere documented. The cytotoicity of blank nanoparticles and cellular uptake of CCC NPs were also investigated.Results. The obtained particles were monodisperse nanospheres with an average hydrated diameter of 151 ± 5.67 nm and surface of charge −22.9 ± 2.21 mV. The MICs of free ciprofloxacin and CCC NPs were 0.16 and 0.08 μg/mL, respectively. Blank nanoparticles showed no obvious cell inhibition within 24 h, and noticeable phagocytosis effect was observed in the presence of CCC NPs.Conclusion. This study shows that CCC NPs have stronger antibacterial activity againstEscherichia colithan the free ciprofloxacin because they can easily be uptaken by cells. The obtained CCC NPs have promising prospect in drug delivery field.

Funder

Liaoning Medical University

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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