Antioxidant and Antiplasmodial Activities of Bergenin and 11-O-Galloylbergenin Isolated fromMallotus philippensis

Abstract

Two important biologically active compounds were isolated fromMallotus philippensis. The isolated compounds were characterized using spectroanalytical techniques and found to be bergenin (1) and 11-O-galloylbergenin (2). Thein vitroantioxidant and antiplasmodial activities of the isolated compounds were determined. For the antioxidant potential, three standard analytical protocols, namely, DPPH radical scavenging activity (RSA), reducing power assay (RPA), and total antioxidant capacity (TAC) assay, were adopted. The results showed that compound2was found to be more potent antioxidant as compared to1. Fascinatingly, compound2displayed better EC50results as compared toα-tocopherol while being comparable with ascorbic acid. The antiplasmodial assay data showed that both the compound exhibited good activity against chloroquine sensitive strain ofPlasmodium falciparum(D10) and IC50values were found to be less than 8 μM. Thein silicomolecular docking analyses were also performed for the determination of binding affinity of the isolated compounds usingP. falciparumproteins PfLDH and Pfg27. The results showed that compound2has high docking score and binding affinity to both protein receptors as compared to compound1. The demonstrated biological potentials declared that compound2could be the better natural antioxidant and antiplasmodial candidate.