Affiliation:
1. College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea
Abstract
After maternal intake, nicotine crosses the placental barrier and causes severe embryonic disorders and fetal death. In this study, we investigated whetherβ-carotene has a beneficial effect against nicotine-induced teratogenesis in mouse embryos (embryonic day 8.5) cultured for 48 h in a whole embryo culture system. Embryos exposed to nicotine (1 mM) exhibited severe morphological anomalies and apoptotic cell death, as well as increased levels of TNF-α, IL-1β, and caspase 3 mRNAs, and lipid peroxidation. The levels of cytoplasmic superoxide dismutase (SOD), mitochondrial manganese-dependent SOD, cytosolic glutathione peroxidase (GPx), phospholipid hydroperoxide GPx, hypoxia inducible factor 1α, andBcl-xL mRNAs decreased, and SOD activity was reduced compared to the control group. However, whenβ-carotene (1×10−7or5×10−7μM)was present in cultures of embryos exposed to nicotine, these parameters improved significantly. These findings indicate thatβ-carotene effectively protects against nicotine-induced teratogenesis in mouse embryos through its antioxidative, antiapoptotic, and anti-inflammatory activities.
Funder
National Research Foundation of Republic of Korea
Subject
Complementary and alternative medicine
Cited by
5 articles.
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