Establishment of Noninvasive Methods for the Detection of Helicobacter pylori in Mongolian Gerbils and Application of Main Laboratory Gerbil Populations in China

Author:

Zhang Xiulin1ORCID,Wang Cunlong1,He Yang12ORCID,Xing Jin3,He Yan4,Huo Xueyun1,Fu Rui3,Lu Xuancheng5,Liu Xin1,Lv Jianyi1,Du Xiaoyan1,Chen Zhenwen1ORCID,Li Changlong1ORCID

Affiliation:

1. Department of Medical Genetics and Developmental Biology, School of Basic Medical Science, Capital Medical University, Beijing Key Laboratory of Cancer Invasion & Metastasis Research, Beijing, China

2. School of Nursing, Dalian Medical University, Dalian, China

3. Institute for Laboratory Animal Resources, National Institutes for Food and Drug Control, Beijing, China

4. Department of Epidemiology and Biostatistics, School of Public Health, Capital Medical University, Beijing, China

5. Laboratory Animal Center, Chinese Center for Disease Control and Prevention, Beijing, China

Abstract

Identifying Helicobacter pylori (H. pylori, Hp) infection in animals before and after artificial infection influences the subsequent experiment. We established effective and noninvasive detection methods, including the gastric fluid nested polymerase chain reaction (PCR) method and the 13C-urea breath test, which can detect Hp before modeling Hp infection in Mongolian gerbils. We designed a gas collection equipment for gerbils. Hp nested PCR was also performed on gastric fluid, gastric mucosa, duodenal contents, and faeces of gerbils challenged with Hp. Conventional Hp detection methods, including rapid urease assay and immunohistochemistry, were compared. Moreover, we assessed the natural infection of Hp in 135 gerbils that had never been exposed to Hp artificially from the major laboratory gerbil groups in China. In 10 Hp infected gerbils, the positive detection results were 100%, 100%, 90%, and 10% in gastric fluid, gastric mucosa, duodenal contents, and faeces with nested PCR, respectively. A rapid urease test performed on gastric mucosa showed that all animals were infected with Hp. Immunohistochemical detection and bacteria culture of gastric mucosa samples that were positive by the nested PCR method also confirmed the presence of Hp. 9% (3/35) and 6% (2/31) natural infection rates were found in conventional gerbil groups from the Capital Medical University and Zhejiang Laboratory Animal Center. In conclusion, we established two noninvasive Hp detection methods that can be performed before modelingHp infection, including the gastric fluid nested PCR method and the 13C-urea breath test.

Funder

Beijing Natural Science Foundation Program and Scientific Research Key Program of Beijing Municipal Commission of Education

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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