Rabdosia japonicavar.glaucocalyxFlavonoids Fraction Attenuates Lipopolysaccharide-Induced Acute Lung Injury in Mice

Abstract

Rabdosia japonicavar.glaucocalyx(Maxim.) Hara, belonging to theLabiataefamily, is widely used as an anti-inflammatory and antitumor drug for the treatment of different inflammations and cancers.Aim of the Study. To investigate therapeutic effects and possible mechanism of the flavonoids fraction ofRabdosia japonicavar.glaucocalyx(Maxim.) Hara (RJFs) in acute lung injury (ALI) mice induced by lipopolysaccharide (LPS).Materials and Methods. Mice were orally administrated with RJFs (6.4, 12.8, and 25.6 mg/kg) per day for 7 days, consecutively, before LPS challenge. Lung specimens and the bronchoalveolar lavage fluid (BALF) were isolated for histopathological examinations and biochemical analysis. The level of complement 3 (C3) in serum was quantified by a sandwich ELISA kit.Results. RJFs significantly attenuated LPS-induced ALI via reducing productions of the level of inflammatory mediators (TNF-α, IL-6, and IL-1β), and significantly reduced complement deposition with decreasing the level of C3 in serum, which was exhibited together with the lowered myeloperoxidase (MPO) activity and nitric oxide (NO) and protein concentration in BALF.Conclusions. RJFs significantly attenuate LPS-induced ALIviareducing productions of proinflammatory mediators, decreasing the level of complement, and reducing radicals.