Expression andIn SilicoAnalysis of the Recombinant Bovine Papillomavirus E6 Protein as a Model for Viral Oncoproteins Studies-Reference-Cited by-同舟云学术

Expression andIn SilicoAnalysis of the Recombinant Bovine Papillomavirus E6 Protein as a Model for Viral Oncoproteins Studies

Published:2013 Issue: Volume:2013 Page:1-9
ISSN:2314-6133
Container-title:BioMed Research International
language:en
Short-container-title:BioMed Research International

Author:

Mazzuchelli-de-Souza J.¹²,Carvalho R. F.¹²,Ruiz R. M.¹²,Melo T. C.¹³,Araldi R. P.¹²,Carvalho E.⁴,Thompson C. E.⁵,Sircili M. P.¹²,Beçak W.¹⁶,Stocco R. C.¹²

Affiliation:

1. Laboratório de Genética, Instituto Butantan, Secretaria de Estado da Saúde, Avenida Vital Brasil, 1500 Butantã, 05503-900 São Paulo, SP, Brazil

2. Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas, Edifício ICB-III-Cidade Universitária, Universidade de São Paulo, Avenida Prof. Lineu Prestes, 2415 Butantã, 05508-900 São Paulo, SP, Brazil

3. Programa de Pós-graduação em Biologia Estrutural e Funcional, Universidade Federal de São Paulo, Rua Botucatu 740, Vila Clementina, 04023-900 São Paulo, SP, Brazil

4. Laboratório de Biotecnologia Molecular, Instituto Butantan, Secretaria de Estado da Saúde, Avenida Vital Brasil 1500, Butantã, 05503-900 São Paulo, SP, Brazil

5. Laboratório Nacional de Computação Científica, Avenida Getúlio Vargas 333, Quitandinha, 25651-075 Petrópolis, RJ, Brazil

6. Departamento de Biologia, Universidade Federal da Integração Latino-Americana (UNILA), Avenida Tancredo Neves 6731 bloco 4, 85867-970 Foz do Iguaçú, PR, Brazil

Abstract

Bovine papillomaviruses (BPVs) are recognized as the causal agents of economical relevant diseases in cattle, associated with the development of tumors in skin and mucosa. The oncogenesis process is mainly associated with different viral oncoprotein expressions, which are involved in cell transformation. The expression and characterization of recombinant viral oncoproteins represent an attractive strategy to obtain biotechnological products as antibodies and potential vaccines, Thus, the aim of this work was to clone and express the BPV-1 and BPV-2 E6 recombinant proteins and performin silicoanalysis in order to develop a strategy for the systematic study of other papillomaviruses oncoproteins. The results demonstrated that BPV-1 and BPV-2 E6 recombinant proteins were expressed and purified from bacterial system as well as itsin silicoanalysis was performed in order to explore and predict biological characteristics of these proteins.

Funder

Ministério de Ciência, Tecnologia e Inovação/Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/bmri/2013/421398.pdf

Reference46 articles.

1. Papillomavirus infections — a major cause of human cancers

2. Human Papillomavirus: Epidemiology, Transmission, and Pathogenesis

3. Expression of SV40 large T antigen, but not small t antigen, is required for the induction of chromosomal aberrations in transformed human cells

4. Analysis of genomic sequences of 95 papillomavirus types: uniting typing, phylogeny, and taxonomy

5. Classification of papillomaviruses

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Pteridium spp. and Bovine Papillomavirus: Partners in Cancer;Frontiers in Veterinary Science;2021-11-02

2. First detection of bovine papillomavirus type 2 in cutaneous wart lesions from ovines;Transboundary and Emerging Diseases;2018-05-03

3. Papillomaviruses: a systematic review;Genetics and Molecular Biology;2017-02-16

4. Bos taurus papillomavirus (BPV) E6 protein: Sequence analysis and molecular evolution;Veterinary Microbiology;2015-12

5. Mutagenic Potential ofBos taurusPapillomavirus Type 1 E6 Recombinant Protein: First Description;BioMed Research International;2015