Hesperidin Inhibits Inflammatory Response Induced byAeromonas hydrophilaInfection and Alters CD4+/CD8+T Cell Ratio

Abstract

Background.Aeromonas hydrophilais an opportunistic bacterial pathogen that is associated with a number of human diseases. Hesperidin (HES) has been reported to exert antioxidant and anti-inflammatory activities.Objectives. The aim of this study was to investigate the potential effect of HES treatment on inflammatory response induced byA. hydrophilainfection in murine.Methods.A. hydrophila-infected mice were treated with HES at 250 mg/kg b.wt./week for 4 consecutive weeks. Phagocytosis, reactive oxygen species production, CD4+/CD8+T cell ratio, and CD14 expression on intestinal infiltrating monocytes were evaluated. The expression of E-selectin and intercellular adhesion molecule 1 on stimulated HUVECs and RAW macrophage was evaluated.Results. Percentage of CD4+T cells in the intestinal tissues of infected treated mice was highly significantly increased; however, phagocytic index, ROS production, CD8+T cells percentage, and CD14 expression on monocytes were significantly reduced. On the other hand, HES significantly inhibited A-LPS- and A-ECP-induced E-selectin and ICAM-1 expression on HUVECs and ICAM-1 expression on RAW macrophage.Conclusion. Present data indicated that HES has a potential role in the suppression of inflammatory response induced byA. hydrophilatoxins through downmodulation of ROS production and CD14 and adhesion molecules expression, as well as increase of CD4+/CD8+cell ratio.