High-Efficiency DNA Extraction Using Poly(4,4′-Cyclohexylidene Bisphenol Oxalate)-Modified Microcrystalline Cellulose-Magnetite Composite

Author:

Al balawi Aisha Nawaf12ORCID,Yusof Nor Azah13ORCID,Kamaruzaman Sazlinda1,Mohammad Faruq4ORCID,Wasoh Helmi5ORCID,Al-Lohedan Hamad A.4ORCID

Affiliation:

1. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

2. Haql College, University of Tabuk, 71491 Tabuk, Saudi Arabia

3. Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

4. Surfactants Research Chair, Department of Chemistry, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia

5. Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

Abstract

In this study, we studied the DNA extraction capability of poly(4,4-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs). The physical characterization techniques like scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA) were employed for the poly(bisphenol Z oxalate)-MCC-magnetite composite during different stages of its formation. The results confirmed the successful modification of the polymer surface. On testing in the presence of three types of binding buffers, a high value of 72.4% (out of 10,000 ng/μL) efficiency with a total yield of DNA at 2×106ng and absorbance ratio of A260/A280 (1.980) was observed for the 2 M GuHCl/EtOH binding buffer. These results were compared against the other two buffers of phosphate-buffered saline (PBS) and NaCl. The lowest value of DNA extraction efficiency at 8125 ng/μL of 58.845% with absorbance ratios of A260/A280 (1.818) for PBS was also observed. The study has concluded an enhancement in the DNA extraction efficiency when the polymer is in the composite stage along with cellulose and magnetite particles as compared against the bare polymer.

Funder

Deanship of Scientific Research, King Saud University

Publisher

Hindawi Limited

Subject

Polymers and Plastics

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