Ackee (Blighia sapida K.D. Koenig) Leaves and Arils Methanolic Extracts Ameliorate CdCl2-Induced Oxidative Stress Biomarkers in Drosophila melanogaster

Author:

Ibraheem Omodele1ORCID,Oyewole Tosin A.1ORCID,Adedara Adeola2ORCID,Abolaji Amos O.2ORCID,Ogundipe Oluwatobiloba M.1ORCID,Akinyelu Jude3ORCID,Eze Chukwuebuka T.4ORCID,Albogami Sarah5,Alotaibi Saqer S.5ORCID,Adeyemi Oluyomi S.6ORCID,Batiha Gaber El-Saber7ORCID,Alorabi Mohammed5ORCID,De Waard Michel8910ORCID

Affiliation:

1. Plants for Biotechnological Resources Research Group, Department of Biochemistry, Federal University Oye-Ekiti, PMB 373, Oye, Ekiti, Nigeria

2. Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Oyo, Nigeria

3. Nanobiochemistry Research Group, Department of Biochemistry, Federal University Oye-Ekiti, PMB 373, Oye, Ekiti, Nigeria

4. Environmental Toxicology Research Group, Department of Biochemistry, Federal University Oye-Ekiti, PMB 373, Oye, Ekiti, Nigeria

5. Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

6. Department of Biochemistry, Medicinal Biochemistry, Nanomedicine & Toxicology Laboratory, Landmark University, PMB, Omu-Aran, 1001, Nigeria

7. Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, El Beheira, Egypt

8. Smartox Biotechnology, 6 rue des Platanes, 38120 Saint-Egreve, France

9. L’Institute du thorax, Inserm, Cnrs, Univ Nantes, F-44007 Nantes, France

10. Universite de Nice Sophia-Antipolis, LabEx Ion Channels, Science and Therapeutics, F-06560, Valbonne, France

Abstract

Different ethnomedical benefits have been documented on different parts of Ackee (Blighia sapida); however, their roles in ameliorating oxidative damages are not well established. CdCl2 inhibitory effects on some oxidative-stress biomarkers and ameliorative potentials of Ackee leaves (AL) and arils (AS) methanolic extracts were studied using Drosophila melanogaster as a model. One to 3-day-old D. melanogaster flies were orally exposed to different concentrations of CdCl2 in their diet for 7 days. The fly’s survival profile and negative geotaxis assays were subsequently analysed. Methanolic extracts of AL and AS treatments showed negative geotaxis behaviour, and extracts were able to ameliorate the effect of Cd2+ on catalase and GST activities and increase total thiol and GSH levels, while it reduced the H2O2 generation ( p 0.05 ) when compared to the control. Furthermore, Cd2+ exhibited noncompetitive and uncompetitive enzyme inhibition on catalase and GST activities, respectively, which may have resulted in the formation of Enzyme-substrate-Cd2+ transition complexes, thus inhibiting the conversion of substrate to product. This study, thus, suggests that the Cd2+ mechanism of toxicity was associated with oxidative damage, as evidenced by the alteration in the oxidative stress-antioxidant imbalance, and that the AL and AS extracts possess essential phytochemicals that could alleviate possibly deleterious oxidative damage effects of environmental pollutants such as CdCl2. Thus, Ackee plant parts possess essential phytonutrients which could serve as valuable resources in heavy metal toxicity management.

Funder

Institut National de la Santé et de la Recherche Médicale

Publisher

Hindawi Limited

Subject

Cell Biology,Aging,General Medicine,Biochemistry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3