In VitroWound Healing Potential and Identification of Bioactive Compounds fromMoringa oleiferaLam

Abstract

Moringa oleiferaLam. (M. oleifera) from the monogeneric familyMoringaceaeis found in tropical and subtropical countries. The present study was aimed at exploring thein vitrowound healing potential ofM. oleiferaand identification of active compounds that may be responsible for its wound healing action. The study included cell viability, proliferation, and wound scratch test assays. Different solvent crude extracts were screened, and the most active crude extract was further subjected to differential bioguided fractionation. Fractions were also screened and most active aqueous fraction was finally obtained for further investigation. HPLC and LC-MS/MS analysis were used for identification and confirmation of bioactive compounds. The results of our study demonstrated that aqueous fraction ofM. oleiferasignificantly enhanced proliferation and viability as well as migration of human dermal fibroblast (HDF) cells compared to the untreated control and other fractions. The HPLC and LC-MS/MS studies revealed kaempferol and quercetin compounds in the crude methanolic extract and a major bioactive compound Vicenin-2 was identified in the bioactive aqueous fraction which was confirmed with standard Vicenin-2 using HPLC and UV spectroscopic methods. These findings suggest that bioactive fraction ofM. oleiferacontaining Vicenin-2 compound may enhance faster wound healingin vitro.