Bacterial Aetiologies of Lower Respiratory Tract Infections among Adults in Yaoundé, Cameroon

Author:

Tchatchouang Serges123,Nzouankeu Ariane2,Kenmoe Sebastien1,Ngando Laure2,Penlap Veronique3,Fonkoua Marie-Christine2,Pefura-Yone Eric-Walter4,Njouom Richard1ORCID

Affiliation:

1. Department of Virology, Centre Pasteur of Cameroon, Member of the International Network of Pasteur Institutes, P.O. Box 1274, Yaoundé, Cameroon

2. Department of Bacteriology, Centre Pasteur of Cameroon, Member of the International Network of Pasteur Institutes, P.O. Box 1274, Yaoundé, Cameroon

3. Department of Biochemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon

4. Department of Pneumology, Jamot Hospital, P.O. Box 4021, Yaoundé, Cameroon

Abstract

Lower respiratory tract infections (LRTIs) remain a challenge in African healthcare settings and only few data are available on their aetiology in Cameroon. The purpose of this study was to access the bacterial cause of LRTIs in patients in Cameroon by two methods.Methods. Participants with LRTIs were enrolled in the referral centre for respiratory diseases in Yaoundé city and its surroundings. To detect bacteria, specimens were tested by conventional bacterial culture and a commercial reverse-transcriptase real-time polymerase chain reaction (RT-PCR) assay. One hundred forty-one adult patients with LRTIs were enrolled in the study. Among the participants, 46.8% were positive for at least one bacterium.Streptococcus pneumoniaeandHaemophilus influenzaewere the most detected bacteria with 14.2% (20/141) followed byKlebsiella pneumoniae, 9.2% (13/141),Staphylococcus aureus, 7.1% (10/141), andMoraxella catarrhalis, 4.3% (6/141). Bacterial coinfection accounted for 23% (14/61) withHaemophilus influenzaebeing implicated in 19.7% (12/61). The diagnostic performance of RT-PCR for bacteria detection (43.3%) was significantly different from that of culture (17.7%) (p< 0.001). OnlyStreptococcus pneumoniaedetection was associated with empyema by RT-PCR (p<0.001). These findings enhance understanding of bacterial aetiologies in order to improve respiratory infection management and treatment. It also highlights the need to implement molecular tools as part of the diagnosis of LRTIs.

Funder

United States Department of Health and Human Services

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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