Clinical Observation of Salvianolic Acid Combined with Panax Notoginseng Saponins Combined with Basic Nursing Intervention on Cerebral Ischemia-Reperfusion Injury in Rats

Abstract

Objective. To analyze the clinical observation of salvianolic acid combined with panax notoginseng saponins combined with basic nursing intervention on cerebral ischemia-reperfusion injury in rats and its effects on the expression of apoptosis-related proteins Bcl-2, Bax and caspase-3. Methods. A total of 72 male Wistar rats were randomly divided into sham, ischemia/reperfusion (I/R), edaravone (Eda), salvianolic acid (SA), panax notoginseng saponins (PNS), and SA+PNS group. After administration for 5 days, the neurological function, cerebral infarction volume, brain index, and brain water content of rats were observed. ELISA kit assay was applied to measure the levels of IL-1β, IL-6, IL-8, TNF-α, MDA, SOD, GSH-Px, and T-AOC activity. Western blotting assay was used to detect the protein levels of p-53, NF-κB, Bcl-2, Bax, and Caspase-3 in the brain tissues surrounding infarction lesion. Results. Compared with sham group, the mNSS score, brain index, brain water content, infarction volumes, MDA activity, and the levels of IL-6, IL-8, TNF-α and IL-1β as well as the protein levels of p-53, NF-κB, Bax and Caspase-3 were significantly increased, while the levels of Bcl-2 protein, SOD, GSH-Px and T-AOC were significantly decreased in I/R group. However, these levels were reversed in SA group, PNS group and SA + PNS group. Moreover, these changes in SA + PNS group were more obvious than those in SA and PNS group, and the differences were statistically significant. Conclusions. SA, PNS and they combined with basic nursing have protective effects on cerebral I/R injury, and the combination with basic nursing has better effects than that used alone. The mechanism may be to regulate the expression of downstream apoptotic proteins by inhibiting the TLR4/NF-κB signaling pathway, thereby reducing neurological damage in rats.