Affiliation:
1. Department of Radiation Oncology, People’s Hospital of Xinjiang Uyghur Autonomous Region, Urumqi, Xinjiang 830001, China
2. Xinjiang Medical University, Urumqi, Xinjiang 830054, China
Abstract
Background. Low molecular heparin (LWMH) therapy can prevent the occurrence of VTE in tumor patients and may have a direct antitumor effect. However, the expression pattern of VEGF-A and microRNAs was less reported in cervical cancer subjects who received concurrent chemoradiotherapy (CCRT) or received anticoagulant treatment with low molecular weight heparin (LWMH) after CCRT (CCRT+LWMH). Methods. In this study, 30 cervical cancer subjects treated with CCRT and 30 cervical cancer patients treated with CCRT+LWMH were enrolled. We screened five miRNAs (miR-15a-5p, miR-16-5p, miR-29a-3p, miR-195-5p, and miR-205-5p), which have multiple binding sites with VEGF-A and are highly expressed in serum of patients with cervical cancer, by RT-qPCR. The expression level of VEGF-A was also detected by RT-qPCR and ELISA. Statistical methods were used for difference and correlation analyses. Results. We observed the curative effect in the two treatment methods. In the CCRT group, the total effective rate was 60.00%, and in the CCRT+LWMT group, the total effective rate was 83.33% (P=0.013, χ2=6.129). Additionally, the serum levels of VEGF-A in the CCRT+LWMH group were downregulated, relative to the CCRT group (P<0.05), and VEGF-A in serum was significantly positively correlated with venous thromboembolism (VTE) (r=2.134, P=0.035). Only miR-205-5p and miR-195-5p were upregulated in CCRT+LWMH, relative to CCRT (P<0.05). In serum of patients with cervical cancer after CCRT+LWMH treatment, there was no significant correlation between VEGF-A and miR-15a-5p (r=−0.132, P=0.209), miR-16-5p (r=−0.205, P=0.311), or miR-29a-3p (r=−0.029, P=0.662), but VEGF-A was significantly negatively correlated with miR-195-5p (r=−0.396, P=0.040) and miR-205-5p (r=−0.315, P=0.032). Furthermore, VTE was also significantly negatively correlated with miR-195-5p (r=−0.412, P=0.031) and miR-205-5p (r=−0.123, P=0.044). Conclusion. These data revealed roles for VEGF-A and these miRNAs as potential biomarkers in cervical cancer patients with VTE, which exhibited usage potential in the treatment of venous thromboembolism.
Funder
Youth Natural Science Foundation of Xinjiang Uyghur Autonomous Region
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
10 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献