A Comparative Histopathology, Serology and Molecular Study, on Experimental Ocular Toxocariasis byToxocara catiin Mongolian Gerbils and Wistar Rats

Author:

Zibaei Mohammad1,Sadjjadi Seyed Mahmoud23,Karamian Mehdi4,Uga Shoji5,Oryan Ahmad6,Jahadi-Hosseini Seyed Hamidreza7

Affiliation:

1. Department of Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, P.O. Box 31485561, Karaj, Iran

2. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, P.O. Box 713451735, Shiraz, Iran

3. Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, P.O. Box 713451735, Shiraz, Iran

4. Department of Microbiology, School of Medicine, Birjand University of Medical Sciences, P.O. Box 917751365, Birjand, Iran

5. Department of Parasitology, Faculty of Health Sciences, Kobe University Graduate School of Health Sciences, P.O. Box 6540142, Kobe, Japan

6. Department of Pathology, School of Veterinary Medicine, Shiraz University, P.O. Box 713451735, Shiraz, Iran

7. Department of Ophthalmology, Khalili Hospital, Shiraz University of Medical Sciences, P.O. Box 713451735, Shiraz, Iran

Abstract

The aim of this study was to compare the performance of three in-house diagnostic tests, that is, histopathology, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR), for the diagnosis after experimental infection withToxocara cati. Twenty Mongolian gerbils and Wistar rats were divided into ten groups (n=2/group).Toxocara catiinfections were established in Mongolian gerbils and Wistar rats by administering doses of 240 and 2500 embryonatedToxocara catieggs by gavage, respectively. Tissue sections were stained with Haematoxylin and Eosin and observed under the light microscope. Sera and vitreous fluid collected from separate infected groups were tested againstToxocara catiantigens, for 92 days postinfection. Genomic DNA was extracted from formalin-fixed paraffin-embedded (FFPE) blocks, and aqueous fluids belong to the animals. The histopathology test gave negative results among the groups of animals examined between 5 and 92 days postinfection. The ELISA results showed that anti-Toxocaraantibodies have risen between 7 and 61 days postinfection in sera and vitreous fluid in the animals infected, respectively. Analysis of PCR products revealed positive band (660 bp) in the orbital tissue infected Mongolian gerbils at 5 days postinfection. Of the three evaluated methods, the PCR could be recommended for scientific and laboratory diagnoses of toxocariasis in experimentally infected animals.

Funder

Shiraz University of Medical Sciences

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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