Ameliorative Effect of Thymoquinone-Loaded PLGA Nanoparticles on Chronic Lung Injury Induced by Repetitive Intratracheal Instillation of Lipopolysaccharide in Rats

Author:

Saghir Sultan A. M.1,Al-Gabri Naif A.234ORCID,Ali Abdelmoniem A.3,Al-Attar Al-Sayed R.3,Al-Sobarry Mosa’d5,Al-shargi Omar Y. A.6,Alotaibi Amal7,Al-zharani Mohammed8,Nasr Fahd A.9ORCID,Al-Balagi Nader10,Abdulghani Mahfoudh A. M.11,Alnaimat Sulaiman M.1,Althunibat Osama Y.1ORCID,Mahmoud Ayman M.1213ORCID

Affiliation:

1. Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma’an 71111, Jordan

2. Department of Pathology, Faculty of Veterinary Medicine, Thamar University, Dhamar 87246, Yemen

3. Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt

4. Laboratory of Djibouti Regional livestock Quarantine, Abu Yasser International Est., Djibouti

5. Department of Pharmacology, College of Pharmacy, Ittihad Private University, Al-Raqqah, Syria

6. College of Pharmacy, Riyadh Elm University, Riyadh, Saudi Arabia

7. Basic Science Department, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia

8. Biology Department, College of Science, Imam Mohammad ibn Saud Islamic University, Riyadh 11623, Saudi Arabia

9. Medicinal, Aromatic and Poisonous Plants Research Center, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia

10. Ministry of Health, Riyadh, Saudi Arabia

11. Department of Pharmacology & Toxicology, Unaizah College of Pharmacy, Qassim University, Al Qassim 51911, Saudi Arabia

12. Biotechnology Department, Research Institute of Medicinal and Aromatic Plants, Beni-Suef University, Beni-Suef, Egypt

13. Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt

Abstract

Thymoquinone (TQ), the active constituent of Nigella sativa, possesses several benefits in traditional and modern medicines. This study examined the effect of a single dose of Nano-TQ on chronic lung injury induced by repetitive intratracheal installation of lipopolysaccharide (LPS). Rats received LPS twice weekly for 8 weeks via intratracheal installation and a single dose of TQ-PLGA NPs on the day after the last dose of LPS. Six rats from each group were sacrificed after 8 and 10 weeks, and samples were collected for analysis. Repetitive intratracheal installation of LPS caused histopathological alterations, including partial or complete obstruction of the alveoli, interstitial edema, mild fibroblastic proliferation, fibrous strands besides lymphocytes and plasma infiltrations, suffered fetalization, bronchiectasis, hypertrophied arterioles, and others. Investigation of the ultrastructure revealed prominent necrotic pneumocytes with destructed chromatin and remnant of necrotic debris in the narrowing alveolar lumen in LPS-induced rats. TQ-PLGA NPs effectively ameliorated LPS-induced histopathological and ultrastructural alterations in the lung of rats. In addition, TQ-PLGA NPs significantly alleviated serum levels of IL-10 and TGF-β1 in LPS-induced rats. In conclusion, TQ-PLGA NPs prevented inflammation and tissue injury in the lungs of rats challenged with repetitive intratracheal installation of LPS. Therefore, TQ-PLGA NPs represent a promising candidate for the prevention of lung injury induced by LPS, pending further studies to determine its safety and exact protective mechanism.

Funder

Princess Nourah bint Abdulrahman University

Publisher

Hindawi Limited

Subject

Cell Biology,Aging,General Medicine,Biochemistry

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