Effects of the Glutamine Administration on T Helper Cell Regulation and Inflammatory Response in Obese Mice Complicated with Polymicrobial Sepsis

Author:

Yeh Chiu-Li1,Su Li-Han1,Wu Jin-Ming23,Yang Po-Jen2,Lee Po-Chu2,Chen Po-Da2,Huang Chun-Chieh24,Hsieh Der-Yirng5,Wang Hsueh-Ju6,Yeh Sung-Ling2,Lin Ming-Tsan2ORCID

Affiliation:

1. School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan

2. Department of Surgery, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan

3. Department of Surgery, National Taiwan University Hospital Hsin-Chu Biomedical Science Park Branch, Hsin-Chu County, Taipei, Taiwan

4. Department of Surgery, National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu County, Taiwan

5. Department of Nursing, Nutrition Support Team, National Taiwan University Hospital, Taipei, Taiwan

6. Department of Pharmacy, National Taiwan University Hospital, Taipei, Taiwan

Abstract

This study investigated the impacts of GLN on inflammation and T cell dysregulation in obese mice complicated with sepsis. Mice were divided into normal control (NC) and high-fat diet groups. The high-fat diet provided 60% of energy from fat and was administered for 10 weeks to induce obesity. Mice fed with a high-fat diet were then assigned to sham (SH) and sepsis with saline (SS) or GLN (SG) groups. The SH group was subjected to laparotomy, while the sepsis group underwent cecal ligation and puncture (CLP). The SS group was intravenously injected with saline. The SG group was intravenously administered GLN after CLP. Mice were sacrificed at 12, 24, or 48 h post-CLP, respectively. Results demonstrated that in the presence of obesity, sepsis drove CD4+ T cells toward the helper T (Th)2 and Th17 lineages. Also, expressions of inflammatory cytokines and macrophage infiltration markers in adipose tissues and lungs were elevated. Treatment of obese mice with GLN after sepsis reversed Th polarization and downregulated macrophage infiltration and inflammatory cytokine, whereas the tight junction-associated protein expression increased in the lungs. These findings suggest that the intravenous administration of GLN to obese mice after sepsis modulated a more balanced Th cell lineage, alleviated inflammation, and attenuated lung injury.

Funder

Taipei Medical University

Publisher

Hindawi Limited

Subject

Cell Biology,Immunology

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