Affiliation:
1. The Affiliated Yuebei People’s Hospital of Shantou University Medical College, Shaoguan, Guangdong 512026, China
2. Shaoguan Institute for Food and Drug Control, Shaoguan, Guangdong 512028, China
Abstract
Objective. To establish the quality standard of Zhiqingshu lotion by high-performance liquid chromatography (HPLC). Methods. HPLC was used to determine emodin, chrysophanol, caffeic acid, and berberine hydrochloride content, key water-soluble components of rhubarb, dandelion, and Phellodendron amurense in Zhiqingshu lotion. The macrophage inflammation model was used to analyze the anti-inflammatory effects of Zhiqingshu lotion. Results. HPLC results showed that the contents of emodin, chrysophanol, caffeic acid, and berberine hydrochloride in Zhiqingshu lotion were
,
,
, and
μg/mL, respectively. Moreover, RT-qPCR results showed that different concentrations of Zhiqingshu lotion significantly reduced the expression of inflammatory factor tumor necrosis factor- (TNF-) α and interleukin- (IL-) 1β in lipopolysaccharide-induced macrophages. Conclusion. HPLC could quantitatively and qualitatively analyze and identify the main components of Zhiqingshu lotion as rhodopsin, rhodopsin, caffeic acid, and berberine hydrochloride. And Zhiqingshu lotion has an excellent anti-inflammatory effect. This method was simple and reliable and could be used for the identification of the ingredients and content of Zhiqingshu lotion, thus improving the quality control of the drug.
Funder
Shaoguan Health Research Project
Subject
Applied Mathematics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Modeling and Simulation,General Medicine
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