Evaluation of an automatic HPLC analyser for thalassemia and haemoglobin variants screening

Abstract

In this paper the authors report the evolution of a new automatic HPLC analyser for screening haemoglobinopathies. HbA2and F determinations are accurate and reproducible. The analysis time is short (6.5 min) and there is a good separation between the HbA2values of β-thalassemia carriers from normals and α-thalassemia carriers, with no overlap between these groups. In addition, the system is also able to detect and quantitate most of the haemoglobin variants, particularly those (HbS, HbC, HbE and Hb Lepore) able to interact with β-thalassemia and could make haemoglobin electrophoresis unnecessary in all samples. The ease of operation and the limited technical work make this system especially suitable for laboratories with a high workload and allow the cost of screening to be reduced.