Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography-Reference-Cited by-同舟云学术

Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography

Published:1992 Issue:4 Volume:14 Page:145-149
ISSN:0142-0453
Container-title:Journal of Automatic Chemistry
language:en
Short-container-title:Journal of Automatic Chemistry

Author:

Ziegler Frédéric¹,Le Boucher Jacques¹,Coudray-Lucas Colette¹,Cynober Luc¹²

Affiliation:

1. Laboratoire de Biochimie, UER des Sciences, Pharmaceutiques et Biologiques, Université Paris XI, Chatenay, 92296, France

2. Laboratoire de Biochimie, UER des Sciences Pharmaceutiques et Biologiques, 5 rue J. B. Clément, Chatenay-Malabry Cedex 92296, France

Abstract

RP-HPLC with automated pre-column OPA derivatization is clearly a suitable alternative for assaying physiological AA and may be particularly useful for AA present at low concentrations (free tryptophan, plasma 3-methylhistidine).

Publisher

Hindawi Limited

Subject

Clinical Biochemistry

Link

http://downloads.hindawi.com/journals/jamc/1992/168020.pdf

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