Lactic Acid Bacteria Strains Exert Immunostimulatory Effect onH. pylori-Induced Dendritic Cells

Abstract

The aim of this study was to find out if selected lactic acid bacteria (LAB) strains (antagonistic or nonantagonistic againstH. pylori in vitro) would differ in their abilities to modulate the DCs maturation profiles reflected by their phenotype and cytokine expression patterns.Methods. Monocyte-derived DCs maturation was elicited by their direct exposure to the LAB strains ofL. rhamnosus900 orL. paracasei915 (antagonistic and nonantagonistic toH. pylori, resp.), in the presence or absence ofH. pyloristraincagA+. The DCs maturation profile was assessed on the basis of surface markers expression and cytokines production.Results. We observed that the LAB strains and the mixtures of LAB withH. pyloriare able to induce mature DCs. At the same time, theL. paracasei915 leads to high IL-10/IL-12p70 cytokine ratio, in contrast toL. rhamnosus900.Conclusions. This study showed that the analyzed lactobacilli strains are more potent stimulators of DC maturation thanH. pylori. Interestingly from the two chosen LAB strains the antagonistic toH. pylori-L. rhamnosusstrain 900 has more proinflammatory and probably antibactericidal properties.