Skew-Laplace and Cell-Size Distribution in Microbial Axenic Cultures: Statistical Assessment and Biological Interpretation

Author:

Julià Olga1,Vidal-Mas Jaume23,Panikov Nicolai S.4,Vives-Rego Josep2

Affiliation:

1. Departament de Probabilitat, Lògica i Estadística, Facultat de Matemàtiques, Universitat de Barcelona, Gran Via, 585, 08007-Barcelona, Spain

2. Departament de Microbiologia, Universitat de Barcelona, Av. Diagonal, 645, 08028 Barcelona, Spain

3. Tres Cantos Medicines Development Campus, GlaxoSmithKline, Severo Ochoa 2, 28760 Tres Cantos, Spain

4. Department of Chemistry & Chemical Biology, Stevens Institute of Technology, Castle Point on Hudson, Hoboken, NJ 07030, USA

Abstract

We report a skew-Laplace statistical analysis of both flow cytometry scatters and cell size from microbial strains primarily grown in batch cultures, others in chemostat cultures and bacterial aquatic populations. Cytometry scatters best fit the skew-Laplace distribution while cell size as assessed by an electronic particle analyzer exhibited a moderate fitting. Unlike the cultures, the aquatic bacterial communities clearly do not fit to a skew-Laplace distribution. Due to its versatile nature, the skew-Laplace distribution approach offers an easy, efficient, and powerful tool for distribution of frequency analysis in tandem with the flow cytometric cell sorting.

Funder

Ministerio de Ciencia y Tecnología

Publisher

Hindawi Limited

Subject

Microbiology (medical),Microbiology

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