Titanium Dioxide Nanoparticles Induced Proinflammation of Primary Cultured Cardiac Myocytes of Rat

Abstract

Titanium dioxide (TiO2) nanoparticles are widely used in electronics, biology, and medicine owing to their special properties. However, during TiO2nanoparticles exposure, nanoparticles may enter the blood circulation and translocate to the heart, and they may result in negative effects on the cardiovascular system. In this study, we demonstrated that the anatase and rutile TiO2nanoparticles had potential toxicological effects on primary cultured cardiac myocytes of rat. After incubating with the anatase and rutile TiO2nanoparticles, the primary cultured cardiac myocytes had become elongated and appeared to detach from the surface of cell plate. After exposure to 50, 100, and 150 μg/mL anatase and rutile TiO2nanoparticles for 2 days, the obvious decrease of cell viability was observed. And further studies showed that TiO2nanoparticles exposure could induce the high expression of proinflammatory cytokines TNF-αand IL-6, especially in 150 μg/mL group. The long-rod rutile TiO2had more strong effects on cell viability and proinflammatory cytokines induction than red-blood cells like anatase TiO2. Results indicated that TiO2nanoparticles exposure could impair the function of primary cultured cardiac myocytes of rat. Therefore, these findings support the view that much more attention should be aroused on the application of these nanoparticles and their potential exposure effects on human beings.