LIN28B Polymorphisms Confer a Higher Postoperative Recurrence Risk in Reproductive-Age Women with Endometrial Polyps

Abstract

The RNA-binding protein LIN28B is an important factor for cell proliferation. Because LIN28B polymorphisms have been shown to be relative with the recurrence of some hyperplastic diseases, we hypothesized that genetic variants of LIN28B gene were associated with postoperative recurrence risk in reproductive-age women with endometrial polyps (EP). In a hospital-based cohort of 351 reproductive female patients underwent hysteroscopic polypectomies between May 2018 and Jan 2020, we genotyped two common polymorphisms in LIN28B gene ( $\text{rs}369065\text{C}>\text{T}$ and $\text{rs}314280\text{A}>\text{G}$ ) and analyzed their associations with the risk of postoperative recurrence in multiple Cox regression model. When followed up to Jun 2021, carries of rs369065 TT genotype had an increased risk of polyp recurrence (adjusting hazard ratio $\left[\text{HR}\right]=1.883$ , 95% confidence interval $\left[\text{CI}\right]=1.033-3.434$ ) and had a shorter time to recurrence (median time 352 vs. 342 days, log-rank $P<0.01$ ), compared to the CC/CT genotype. Further stratification analysis showed that the increased risk of rs369065 TT genotype was more evident in patients who were older than 33 years (adjusted $\text{HR}=2.597$ , $95\%\text{CI}=1.037-6.505$ ), had a single polyp (adjusted $\text{HR}=2.545$ , $95\%\text{CI}=1.059-6.113$ ), and had smaller polyps (<1.2 cm, adjusted $\text{HR}=2.708$ , $95\%\text{CI}=1.042-7.043$ ). However, no significant association between $\text{rs}314280\text{A}>\text{G}$ polymorphism and the risk of polyp recurrence was found. Our study suggests that rs369065 TT genotype of LIN28B gene is associated with an increased postoperative recurrence risk in EP patients, especially in those with fewer and smaller polyps. These findings implicate a precise choice of clinical counseling and decision making. Larger studies in different ethnic populations are warranted.