Evaluation of Genotoxic Effect and Antigenotoxic Potential against DNA Damage of the Aqueous and Ethanolic Leaf Extracts of Annona muricata Using an In Vivo Erythrocyte Rodent Micronucleus Assay-Reference-Cited by-同舟云学术

Evaluation of Genotoxic Effect and Antigenotoxic Potential against DNA Damage of the Aqueous and Ethanolic Leaf Extracts of Annona muricata Using an In Vivo Erythrocyte Rodent Micronucleus Assay

Published:2022-12-16 Issue: Volume:2022 Page:1-15
ISSN:2314-6141
Container-title:BioMed Research International
language:en
Short-container-title:BioMed Research International

Author:

Sánchez-De-La-Rosa Susana Vanessa¹^ORCID,Lazalde-Ramos Blanca Patricia²^ORCID,Morales-Velazquez Gabriela¹^ORCID,Zúñiga-González Guillermo Moisés³^ORCID,Gómez-Meda Belinda Claudia⁴^ORCID,Sánchez-Rivera Saulo Oswaldo¹^ORCID,Ortiz-García Yveth Marlene¹^ORCID,Guerrero-Velazquez Celia¹^ORCID,Zamora-Perez Ana Lourdes¹^ORCID

Affiliation:

1. Instituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Mexico

2. Maestría en Ciencias y Tecnología Química, Unidad Académica de Ciencias Químicas, Universidad Autónoma de Zacatecas, Zacatecas 98000, Mexico

3. Laboratorio de Mutagenesis, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara 44340, Mexico

4. Instituto de Genética Humana “Enrique Corona Rivera” Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara 44340, Mexico

Abstract

Annona muricata have been extensively used in traditional medicine to treat multiple diseases, including cancers. This study evaluated the genotoxic potential and antigenotoxic activities of A. muricata aqueous and ethanolic leaf extracts by employing an in vivo erythrocyte rodent micronucleus assay. Different doses (187.5, 375, and 750 mg/kg) of both extracts were administered orally for 5 days alone and combined with cyclophosphamide (CP, 60 mg/kg) to BALB/c mice. Also, it was administered orally to Wistar rats for 5 days through the final stage of gestation. No genotoxic or cytotoxic effects were observed in the two adult rodent models when A. muricata was administered orally nor in newborn rats transplacentally exposed to the extracts. Moreover, A. muricata aqueous and ethanolic leaf extracts demonstrated a protective effect against CP-induced DNA damage. Due to its lack of genotoxic effect and its capacity to decrease DNA damage, A. muricata is likely to open an interest field regarding its potential safe use in clinical applications.

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://downloads.hindawi.com/journals/bmri/2022/9554011.pdf

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