Detection of the Content of Two Coumarins, IM and ISOIM, and Their Mechanism of Action on Colitis Rats in Angelica albicans

Abstract

Angelica albicans is being used in the cure of different, respiratory, neuromuscular, and cutaneous diseases in traditional eastern medicine. The pharmacokinetic (PK) characteristics of imperatorin (IM) and isoimperatorin (ISOIM), the main effective components in Angelica albicans, were investigated. The rapid, subtle, and measuring the PKs of a drug, a validated UPLC/MS/MS methodology was designed for a total of 2 furanocoumarins in 2,4,6-trinitrobenzene sulfonic acid-stimulated and untreated mice. After that, blood samples were obtained. Angelica albicans (0.5 and 1.0 g/kg) was given orally, taken regularly from the tail vein. The time it takes for colitis rats to achieve their maximal concentration ( $T_{\max }$ ) imperatorin and isoimperatorin was considerably postponed. In comparison to normal rats, all furanocoumarins had lesser peak plasma concentrations ( $C_{\max }$ ) and higher represent residence durations. The area below the $C_{\max }$ time-curve or clearance half-life did not differ significantly. In normal rats, all two furanocoumarins attained maximal plasma levels between 40 and 75 minutes, demonstrating fast oral absorption. The periods to attain $T_{\max }$ of the two furanocoumarins, on the other hand, were shorter than in earlier studies. Therefore, colitis-linked alterations in the drug-absorption stage may result in a late $T_{\max }$ and lowered $C_{\max }$ , which have no effect on its clearance in half-life. Hence, conclusively, as a result, more consideration should be given to the prescription and administration of Angelica albicans in colitis individuals, and more research is needed to determine whether the changed PK profile was clinically meaningful for medicinal dose.