Time-Evolution Contrast of Target MRI Using High-Stability Antibody Functionalized Magnetic Nanoparticles: An Animal Model-Reference-Cited by-同舟云学术

Time-Evolution Contrast of Target MRI Using High-Stability Antibody Functionalized Magnetic Nanoparticles: An Animal Model

Published:2014 Issue: Volume:2014 Page:1-7
ISSN:1687-4110
Container-title:Journal of Nanomaterials
language:en
Short-container-title:Journal of Nanomaterials

Author:

Huang K. W.¹²,Yang S. Y.³,Horng H. E.⁴,Chieh J. J.⁴,Chen H. H.⁴,Wu C. C.⁵⁶,Chen J. H.⁷,Lin I.T.⁸⁹,Yang C. C.³,Yang H. C.¹⁰

Affiliation:

1. Department of Surgery & Hepatitis Research Center, National Taiwan University Hospital, Taipei 100, Taiwan

2. Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan

3. MagQu Co., Ltd., New Taipei City 231, Taiwan

4. Institute of Electro-Optical Science and Technology, National Taiwan Normal University, Taipei 116, Taiwan

5. Department of Internal Medicine, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei 100, Taiwan

6. Department of Primary Care Medicine, National Taiwan University, Taipei 100, Taiwan

7. Department of Material Engineering, Kun Shan University, Tainan City 710, Taiwan

8. Center for Molecular Imaging and Translational Medicine, Xiamen University, Xiamen 361, China

9. Graduate Institute of Electronics Engineering, National Taiwan University, Taipei 106, Taiwan

10. Department of Electro-Optical Engineering, Kun Shan University, Tainan City 710, Taiwan

Abstract

In this work, high-quality antibody functionalized Fe3O4magnetic nanoparticles are synthesized. Such physical characterizations as particle morphology, particle size, stability, and relaxivity of magnetic particles are investigated. The immunoreactivity of biofunctionalized magnetic nanoparticles is examined by utilizing immunomagnetic reduction. The results show that the mean diameter of antibody functionalized magnetic nanoparticles is around 50 nm, and the relaxivity of the magnetic particles is 145 (mM·s)−1. In addition to characterizing the magnetic nanoparticles, the feasibility of using the antibody functionalized magnetic nanoparticles for the contrast medium of target magnetic resonance imaging is investigated. These antibody functionalized magnetic nanoparticles are injected into mice bearing with tumor. The tumor magnetic-resonance image becomes darker after the injection and then recovers 50 hours after the injection. The tumor magnetic-resonance image becomes the darkest at around 20 hours after the injection. Thus, the observing time window for the specific labeling of tumors with antibody functionalized magnetic nanoparticles was found to be 20 hours after injecting biofunctionalized magnetic nanoparticles into mice. The biopsy of tumor is stained after the injection to prove that the long-term darkness of tumor magnetic-resonance image is due to the specific anchoring of antibody functionalized magnetic nanoparticles at tumor.

Funder

Ministry of Science and Technology, Taiwan

Publisher

Hindawi Limited

Subject

General Materials Science

Link

http://downloads.hindawi.com/journals/jnm/2014/351848.pdf

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