Predominant Dissemination of PVL-Negative CC89 MRSA with SCCmecType II in Children with Impetigo in Japan

Abstract

Background. The ratio of CA-MRSA in children with impetigo has been increasing in Japan.Methods. Antimicrobial susceptibilities of 136S. aureusisolates from children with impetigo were studied. Furthermore, molecular epidemiological analysis and virulence gene analysis were performed.Results. Of the 136S. aureusisolates, 122 (89.7%) were MSSA and 14 (10.3%) were MRSA. Of the 14 MRSA strains, 11 belonged to CC89 (ST89, ST91, and ST2117) and carried diverse types of SCCmec: type II (IIb: 3 strains; unknown subtype: 4 strains), type IVa (2 strains), and unknown type (2 strains). The remaining three strains exhibited CC8 (ST-8)-SCCmectype VIa, CC121 (ST121)-SCCmectype V, and CC5 (ST5)-nontypeable SCCmecelement, respectively. None werelukS-PV-lukF-PV gene positive. Gentamicin- and clarithromycin-resistant strains were frequently found in both MRSA and MSSA.Conclusions. PVL-negative CC89-SCCmectype II strains are the most predominant strains among the CA-MRSA strains circulating in the community in Japan.