Ochratoxin A Control in Meat Derivatives: Intraspecific Biocompetition betweenPenicillium nordicumStrains

Abstract

APenicillium nordicumstrain previously assessed for its atoxigenicity was tested against a toxigenic strain of the same species on salami, in order to assess its effectiveness as a biocontrol agent for OTA containment. Sixtysalamiwere inoculated with different combinations ofP. nordicumOTA−/OTA+ suspensions and ripened under controlled thermohygrometric conditions. After 7, 18, 29, and 40 days, both fungal counts and chemical analyses were carried out on casings. OTA was never found insalamiused as a control, while it was occasionally detected in traces (0.08–0.76 μg/kg) insalamiinoculated with the atoxigenicP. nordicumstrain. It was otherwise detected at levels varying from 2.84 to 15.85 μg/kg in coinoculated salami and from 48.66 to 177.79 μg/kg insalamiinoculated with the toxigenicP. nordicumstrain. OTA levels detected when coinoculation occurred were 91.1%, 85.8%, and 94.2% lower than those found in samples inoculated with the toxigenic strain, respectively, after 18, 29, and 40 ripening days. Biocontrol approach using intraspecific competition proved very effective in reducing both settlement of toxigenic strains and OTA contamination and could be therefore considered an interesting strategy to avoid OTA contamination in moulded meats, if used in association with fungal commercial starters.