The Potential Use of Platelet-Rich Plasma to Reconstruct the Microtia Chondrocyte in Human Auricular Cartilage Regeneration

Author:

Chen Wei-Hong12,Liu Hen-Yu12ORCID,Tsai Ching-Yu12,Wu Chia-che3,Wei Hong-Jian12,Liu Alice12,Lai Ming-Tang3,Huang Chiung-Fang4,Deng Win-Ping12

Affiliation:

1. Stem Cell Research Center, Taipei Medical University, Taipei 110, Taiwan

2. Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan

3. Department of Otolaryngology, Wan-Fang Hospital, Taipei Medical University, Taipei 116, Taiwan

4. Department of Dentistry, Taipei Medical University Hospital, Taipei 110, Taiwan

Abstract

Microtia is characterized as an incomplete auricular development and surgical reconstruction for microtia is still limited even with emerging developments. This study aimed to apply bionanomaterials (PRP/collagen scaffold) for human auricular neocartilage reconstruction by using microtia chondrocytes. The results showed that PRP (TGF-β1 750 pg/mL and 1 ng/mL) increased cell viability of microtia chondrocytes during in vitro 9-day cultures. Additionally, chondrogenic-specific mRNA of Aggrecan and type II collagen (Col II) was significantly and continuously expressed with PRP treatment during the 21-day in vitro expansion. Tissue engineering of auricular neocartilage was performed by seeding microtia chondrocytes in bionanomaterials (PRP/collagen scaffold) 3-dimensional (3D) cultures. Immunohistochemistry (IHC) of Col II showed intensive signals between cells and matrix after 4-week cultures. Conclusion. Our results demonstrated that PRP promotes proliferation and redifferentiation of microtia chondrocytes and provides regenerative potentials in auricular neocartilage reconstruction.

Publisher

Hindawi Limited

Subject

General Materials Science

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Porous biomaterials for tissue engineering: a review;Journal of Materials Chemistry B;2022

2. Ear Tissue Engineering;Comprehensive Biotechnology;2019

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