Affiliation:
1. Microbiology and Immunology, Kirksville College of Osteopathic Medicine, A.T. Still University of Health Sciences, 800 West Jefferson Street, Kirksville, MO 63501, USA
Abstract
Nitric oxide and its derivative peroxynitrites are generated by host defense system to control bacterial infection. However certain Gram positive bacteria includingStaphylococcus aureuspossess a gene encoding nitric oxide synthase (SaNOS) in their chromosome. In this study it was determined that under normal growth conditions, expression ofSaNOSwas highest during early exponential phase of the bacterial growth. In oxidative stress studies, deletion ofSaNOSled to increased susceptibility of the mutant cells compared to wild-typeS. aureus. While inhibition ofSaNOSactivity by the addition of L-NAME increased sensitivity of the wild-typeS. aureusto oxidative stress, the addition of a nitric oxide donor, sodium nitroprusside, restored oxidative stress tolerance of theSaNOSmutant. TheSaNOSmutant also showed reduced survival after phagocytosis by PMN cells with respect to wild-typeS. aureus.
Funder
National Institutes of Health
Subject
Microbiology (medical),Microbiology
Cited by
16 articles.
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