Human Platelet-Rich Plasma Regulates Canine Mesenchymal Stem Cell Migration through Aquaporins

Author:

Parascandolo Alessia12ORCID,Di Tolla Michele Francesco1ORCID,Liguoro Domenico12,Lecce Manuela1,Misso Saverio3,Micieli Fabiana4ORCID,Ambrosio Maria Rosaria12ORCID,Cabaro Serena12ORCID,Beguinot Francesco12,Pelagalli Alessandra56ORCID,D’Esposito Vittoria12ORCID,Formisano Pietro12ORCID

Affiliation:

1. Department of Translational Medical Sciences, University of Naples “Federico II”, Via Pansini 5, 80131 Naples, Italy

2. URT “Genomic of Diabetes”, Institute for Experimental Endocrinology and Oncology “G. Salvatore”, National Research Council (IEOS-CNR), Via Pansini 5, 80131 Naples, Italy

3. Unit of Transfusion Medicine, Azienda Sanitaria Locale Caserta, Caserta, Italy

4. Department of Veterinary Medicine and Animal Productions, University of Napoli Federico II, 80137 Naples, Italy

5. Department of Advanced Biomedical Sciences, University of Napoli Federico II, 80131 Naples, Italy

6. Institute of Biostructures and Bioimages, National Research Council, 80145 Naples, Italy

Abstract

Platelet products are commonly used in regenerative medicine due to their effects on the acceleration and promotion of wound healing, reduction of bleeding, synthesis of new connective tissue, and revascularization. Furthermore, a novel approach for the treatment of damaged tissues, following trauma or other pathological damages, is represented by the use of mesenchymal stem cells (MSCs). In dogs, both platelet-rich plasma (PRP) and MSCs have been suggested to be promising options for subacute skin wounds. However, the collection of canine PRP is not always feasible. In this study, we investigated the effect of human PRP (hPRP) on canine MSCs (cMSCs). We isolated cMSCs and observed that hPRP did not modify the expression levels of the primary class of major histocompatibility complex genes. However, hPRP was able to increase cMSC viability and migration by at least 1.5-fold. hPRP treatment enhanced both Aquaporin (AQP) 1 and AQP5 protein levels, and their inhibition by tetraethylammonium chloride led to a reduction of PRP-induced migration of cMSCs. In conclusion, we have provided evidence that hPRP supports cMSC survival and may promote cell migration, at least through AQP activation. Thus, hPRP may be useful in canine tissue regeneration and repair, placing as a promising tool for veterinary therapeutic approaches.

Funder

Associazione Italiana per la Ricerca sul Cancro

Publisher

Hindawi Limited

Subject

Cell Biology,Molecular Biology

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