miR-1266-3p Suppresses Epithelial-Mesenchymal Transition in Colon Cancer by Targeting P4HA3

Author:

Zhou Hailang12ORCID,Huang Shu2ORCID,Shao Changjiang3ORCID,Zou Junwei4ORCID,Zhou Aijun2ORCID,Yu Jiufeng5ORCID,Xu Chunfang1ORCID

Affiliation:

1. Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China

2. Department of Gastroenterology, Lianshui People’s Hospital Affiliated to Kangda College of Nanjing Medical University, Huaian, Jiangsu 223400, China

3. Department of Gastroenterology, The Second People’s Hospital of Lianyungang, Lianyungang, Jiangsu 222006, China

4. Department of General Surgery, The Second Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China

5. Department of Traditional Chinese Medicine, Lianshui People’s Hospital Affiliated to Kangda College of Nanjing Medical University, Huaian, Jiangsu 223400, China

Abstract

Numerous studies have been conducted to demonstrate that miRNA is strongly related to colon cancer progression. Nevertheless, there are few studies regarding the function for miR-1266-3p in colon cancer, and the molecular mechanism remains poorly know. Our study was designed to examine the level of miR-1266-3p expression among the colon cancer tissue and cell and to study the role and regulatory mechanism for miR-1266-3p among colon cancer’s malignant biologic behavior. First, we found that miR-1266-3p expression was distinctly lower in colonic carcinoma tissues and cells than in nontumor ones, and the prognosis of low miR-1266-3p patients was distinctly worse than that of high miR-1266-3p patients. Second, we predicted that the target gene of miR-1266-3p was prolyl 4-hydroxylase subunit alpha 3 (P4HA3) through bioinformatics, and the targeting relationship between the two was verified by a dual luciferase assay report. Furthermore, miR-1266-3p inhibited the growth and metastasis of colon cancer in vitro as well as in vivo, and this effect could be alleviated by overexpressing P4HA3. Even more importantly, our study demonstrated that miR-1266-3p inhibited epithelial-mesenchymal transition (EMT) by targeting P4HA3. In conclusion, miR-1266-3p could inhibit growth, metastasis, and EMT in colon cancer by targeting P4HA3. Our discoveries might offer a novel target for colon cancer diagnosis and treatment.

Funder

Primary Research & Social Development Plan of Jiangsu Province

Publisher

Hindawi Limited

Subject

Cancer Research,Cell Biology,Molecular Medicine,General Medicine,Pathology and Forensic Medicine

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