Affiliation:
1. Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana 136119, India
2. Department of Pharmacy, School of Chemical Sciences and Pharmacy, Central University of Rajasthan, Ajmer 305801, India
Abstract
Aims. This study was designed to investigate the enzyme inhibitory and antidiabetic activity for the constituents isolated fromDillenia indica.Methods. The leaves ofD. indicawere extracted with methanol and subjected to fractionation and chromatographic separation, which led to the isolation of seven compounds: betulinic acid (1), n-heptacosan-7-one (2), n-nonatriacontan-18-one (3), quercetin (4),βsitosterol (5), stigmasterol (6), and stigmasteryl palmitate (7). Among these isolates, compounds1,4,5, and6were evaluated forin vitroenzyme inhibition and compounds4,5and6were evaluated for antidiabetic activity in streptozotocin-nicotinamide induced diabetic mice.Results. Compounds1,4,5, and6showed 47.4, 55.2, 48.8, and 44.3%α-amylase inhibition, respectively, and 52.2, 78.2, 52.5, and 34.2%α-glucosidase inhibition, respectively, at the dose of 50 µg/kg. Compounds4,5and6also showed significant (*P<0.05) antidiabetic activity in streptozotocin-nicotinamide induced diabetic mice at the dose of 10 mg/kg.Conclusion.These results provide evidence thatDillenia indicamight be a potential source of antidiabetic agents.
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
120 articles.
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