Role of the Transient Receptor Potential Vanilloid Type 1 (TRPV1) in the Regulation of Nitric Oxide Release in Wistar Rat Aorta

Author:

Varela-López Elvira1ORCID,del Valle-Mondragón Leonardo2ORCID,Castrejón-Téllez Vicente3ORCID,Pérez-Torres Israel4ORCID,Arenas Araceli Páez1ORCID,Rojas Felipe Massó1ORCID,Guarner-Lans Verónica3ORCID,Vargas-González Alvaro3ORCID,Pastelín-Hernández Gustavo2ORCID,Torres-Narváez Juan Carlos2ORCID

Affiliation:

1. Laboratorio de Cardiología Translacional, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, 14080 Tlalpan, Ciudad de México, Mexico

2. Departamento de Farmacología “Dr. Rafael Méndez Martínez”, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, 14080 Tlalpan, Ciudad de México, Mexico

3. Departamento de Fisiología, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, 14080 Tlalpan, Ciudad de México, Mexico

4. Departamento of Biomedicina Cardiovascular, Instituto Nacional de Cardiología “Ignacio Chávez”, Juan Badiano 1, Sección XVI, 14080 Tlalpan, Ciudad de México, Mexico

Abstract

The potential transient vanilloid receptor type 1 (TRPV1) plays important functional roles in the vascular system. In the present study, we explored the role of the TRPV1 in the production of nitric oxide (NO), biopterines (BH4 and BH2), cyclic guanosine monophosphate (cGMP), malondialdehyde (MDA), phosphodiesterase-3 (PDE-3), total antioxidant capacity (TAC), and calcitonin gene-related peptide (CGRP) in the rat aorta. Wistar rats were divided into four groups: (1) control, (2) capsaicin (CS, 20 mg/kg), (3) capsazepine (CZ, 24 mg/kg), and (4) CZ + CS. Treatments were applied daily for 4 days before removing the thoracic aortas for testing of aortic tissue and endothelial cells. TRPV1 activation produced increases in BH4 14%, cGMP 25%, NO 29%, and TAC 59.2% in comparison to the controls. BH2 and MDA increased with CZ. CGRP shows a tendency to decrease with CZ. The analysis by immunocytochemistry confirmed that the TRPV1 is present in aortic endothelial cells. Aortic endothelial cells were obtained from healthy rats and cultured to directly explore the effects of CS and CZ. The activation of the TRPV1 (CS 30 μM) produced increases in BH4 17%, NO 36.6%, TAC 56.3%, and CGRP 65%, when compared to controls. BH2 decreased with CZ + CS. CS effects were diminished by CZ in cells and in the tissue. We conclude that the TRPV1 is a structure present in the membrane of aortic endothelial cells and that it participates in the production of NO. The importance of the TRPV1 should be considered in vascular reactivity studies.

Publisher

Hindawi Limited

Subject

Cell Biology,Aging,General Medicine,Biochemistry

Reference30 articles.

1. Endothelial Dysfunction: Clinical Implications in Cardiovascular Disease and Therapeutic Approaches

2. Thoracic aortic aneurysm development in patients with bicuspid aortic valve: what is the role of endothelial cells?;V. van de Pol;Frontiers in Physiology,2017

3. Lipid peroxidation-derived aldehydes, 4-hydroxynonenal and malondialdehyde in aging-related disorders;G. Barrera;Antioxidants,2018

4. The capsaicin receptor: a heat-activated ion channel in the pain pathway;M. J. Caterina;Nature,1997

5. Capsaicinoids in the treatment of neuropathic pain: a review

Cited by 3 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3