Effect of Intervention of Probiotics in Advance on Treg/Th17 in Premature Mice

Abstract

To preliminarily understand the differentiation characteristics of regulatory T cells (Tregs) and Th17 at a different time in preterm mice, the impacts of probiotics on immune function progression, as well as the correlation of probiotics with Tregs and Th17. On embryonic day 18 of gestation, a mouse model of preterm birth was built using mifepristone (RU486). Following IPI of RU486, newborn mice were randomized to probiotics or NS gavage administration. Full-term newborn mice were given the same dose of NS gavage administration. Phenotypic analysis of peripheral immune cell frequency was performed using flow cytometry. Cytokine measurements were phenotyped by enzyme-linked immunosorbent assays. On the 14th and 21st days after birth, the highest and lowest expressions of Foxp3, the Treg transcription factor, were observed in full-term mice and premature mice by NS gavage administration, respectively, while the opposite trend was found in the Th17 transcription factor IL-17.IL-2, IL-6, and TGF-β rose with age but showed different trends among the three groups. IL-2 is the highest in full-term mice and the lowest in premature mice. IL-6 and TGF-β is the lowest in full-term mice and the highest in premature mice. Probiotics are beneficial to the development and maturation of the immune system, which may play a role in regulating the ratio of Treg/Th17. Probiotic preintervention can effectively promote the differentiation of Treg and inhibit the differentiation of Th17 in premature mice. Its mechanism of action may play a biological role by regulating cytokine (IL-2, IL-6, and TGF-β) secretions.