In Vitro Antiviral Activity of Green Tea Polyphenon-60 against Avian Paramyxoviruses

Abstract

Avian paramyxoviruses (APMVs) have caused an economically significant drop in global domestic poultry production because of their high morbidity and mortality rates. Polyphenols are the major components of green tea that have great antiviral effects. This study aimed to evaluate the anti-APMV activities of polyphenon-60. Twelve APMV-1 strains representing three different pathotypes, two strains of APMV-2, one strain of APMV-3, and one strain of APMV-7 were propagated in chicken embryos. To determine the cytotoxic effect, chicken embryo fibroblasts were treated with the test compound in various concentrations. To assess the antiviral properties, time-dependent, dose-dependent, and virulence-dependent experiments were conducted in both cell and chicken embryo models. A reduction in virus titers was measured by the hemagglutination test. The inhibitory effect on virus adsorption to the chicken red blood cell (RBC) surface was examined by the hemagglutination inhibition test. The results showed that lentogenic and mesogenic APMV-1 strains, APMV-3 strain, and APMV-7 strain were significantly inhibited ( $p<0.05$ ) by polyphenon-60 at 50 μg/ml, while the 50% cytotoxic concentration of the compound was 345 μg/ml. Polyphenon-60 also exhibited the inhibitory activity against hemagglutination by NDV. Taken together, the results suggest that polyphenon-60 has shown promise as an antiviral agent that has wide safety margins against APMVs, and challenge studies to evaluate its efficacy in chickens are necessary.