Global Single-Cell Sequencing Landscape of Adipose Tissue of Different Anatomical Site Origin in Humans

Author:

Gu Shixing1,Gong Zhenyu2,Liu Shuncai2,Lu Guohao3,Ling Yu3,Wei Yanlin3,Li Ting4,Gu Ronghe5,Rong Yongxian6ORCID,Li Junjun7ORCID,Li Hongmian8ORCID

Affiliation:

1. Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533022 Guangxi, China

2. Department of Burn, Plastic and Aesthetic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, 541001 Guangxi, China

3. Department of Emergency, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi 53002, China

4. Department of Basic Science, YuanDong International Academy of Life Sciences, Hong Kong 999077, China

5. Department of Orthopedics, The Fifth Affiliated Hospital of Guangxi Medical University & The First People’s Hospital of Nanning, Nanning, 53002 Guangxi, China

6. Department of Burn, Plastic and Aesthetic Surgery, The Guiping People's Hospital, Guigping, 537200, China

7. Department of Pediatrics, The People’s Hospital of Guangxi Zhuang Autonomous Region & Institute of Hospital Management and Medical Prevention Collaborative Innovation, Guangxi Academy of Medical Sciences, Nanning, 530021 Guangxi, China

8. Department of Plastic and Reconstructive Surgery, The People’s Hospital of Guangxi Zhuang Autonomous Region & Research Center of Medical Sciences, Guangxi Academy of Medical Sciences, Nanning, 530021 Guangxi, China

Abstract

Chronic refractory wounds (CRW) are one of the most serious clinical challenges for surgeons to address. Stromal vascular fraction gels (SVFG), including human adipose stem cells (hASCs), have excellent vascular regenerative and tissue repair properties. Here, we combined single-cell RNA sequencing (scRNA-seq) of leg subcutaneous adipose tissue samples with scRNA-seq data from abdominal subcutaneous adipose tissue, leg subcutaneous adipose tissue, and visceral adipose tissue samples from public databases. The results showed specific differences in cellular levels in adipose tissue from different anatomical site sources. We identified cells including CD4+ T cells, hASCs, adipocyte (APC), epithelial (Ep) cells, and preadipocyte. In particular, the dynamics between groups of hASCs, epithelial cells, APCs, and precursor cells in adipose tissue of different anatomical site origins were more significant. Furthermore, our analysis reveals alterations at the cellular level and molecular level, as well as the biological signaling pathways involved in these subpopulations of cells with specific alterations. In particular, certain subpopulations of hASCs have higher cell stemness, which may be related to lipogenic differentiation capacity and may be beneficial in promoting CRW treatment and healing. In general, our study captures a human single-cell transcriptome profile across adipose depots, the cell type identification and analysis of which may help dissect the function and role of cells with specific alterations present in adipose tissue and may provide new ideas and approaches for the treatment of CRW in the clinical setting.

Funder

National Natural Science Foundation of China

Publisher

Hindawi Limited

Subject

Cell Biology,Molecular Biology

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