Budding ofTaenia crassicepsCysticerci In Vitro Is Promoted by Crowding in Addition to Hormonal, Stress, and Energy-Related Signals

Author:

Ostoa-Saloma Pedro1,Ostoa-Jacobo Pedro1,Esquivel-Velázquez Marcela1,Bazúa Silvana1,Larralde Carlos1

Affiliation:

1. Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, C.P. 04510, México D.F., Mexico

Abstract

Taenia crassicepscysticerci (cysts) reproduce by budding. The cysts' production of buds was measured in vitro to explore parasite and environmental-related factors involved in the extreme individual variation in parasite loads of inbred mice. Cysts were placed in in vitro culture for 10 days at initial parasite densities of 1, 5, 10 cysts/well in 1 ml of RPMI Medium 1640 without serum. Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts. Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17ß-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources. All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.

Funder

Consejo Nacional de Ciencia y Tecnología

Publisher

Hindawi Limited

Subject

Health, Toxicology and Mutagenesis,Genetics,Molecular Biology,Molecular Medicine,General Medicine,Biotechnology

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