Improved Thrombin Hemostat Using the Cross-Linked Gelatin by Microbial Transglutaminase

Abstract

It is well known that the thrombin obtained from animal is available in clinical treatment, which plays an important role in hemostasis and the treatment of hemorrhagic diseases. However, how to achieve complete hemostasis in 2 min is still a challenge. In this report, the thrombin hemostatic has been improved using the cross-linked gelatin by microbial transglutaminase, and its efficacy was demonstrated by the vitro experiment. Compared with the traditional thrombin hemostatic the clotting time with the improved hemostat is significantly shorter. It may rapidly stop blood loss, which would provide a simple, safe, and cost-effective surgical sealant.