Identification of Species of Nontuberculous Mycobacteria Clinical Isolates from 8 Provinces of China

Abstract

Pulmonary diseases caused by nontuberculousmycobacteria(NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces in China, in order to preliminarily provide some basic scientific data in the different species and distribution of NTM related to pulmonary disease in China. A total of 523 clinical isolates from patients with tuberculosis (TB) diagnosed clinically from 2005 to 2012 were identified to the species using conventional and molecular methods, including multilocus PCR,rpoBandhsp65PCR-PRA,hsp65,rpoB, and16S-23Sinternal transcribed spacer region sequencing. The isolates were identified into 3 bacterium genera, including NTM,Gordonia bronchialis,andNocardia farcinica, and, for the 488 NTM isolates, 27 species were identified. For all the 27 species of NTM which were found to cause pulmonary infections in humans, the most prevalent species wasM. intracellulare, followed byM. aviumandM. abscessus. And seven other species were for the first time identified in patients with TB in China. NTM species identification is very important for distinguishing between tuberculosis and NTM pulmonary diseases, and the species diversity drives the creation of diverse and integrated identification methods with higher accuracy and efficacy.