Constructing Physical and Genomic Maps forPuccinia striiformisf. sp.tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence ofP. graminisf. sp.tritici, the Wheat Stem Rust Pathogen

Author:

Ma Jinbiao12,Chen Xianming23,Wang Meinan2,Kang Zhensheng4

Affiliation:

1. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China

2. Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA

3. USDA-ARS, Wheat Genetics Quality, Physiology, and Disease Research Unit, Pullman, WA 99164-6430, USA

4. College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China

Abstract

The wheat stripe rust fungus,Puccinia striiformisf. sp.tritici(Pst), does not have a known alternate host for sexual reproduction, which makes it impossible to study gene linkages through classic genetic and molecular mapping approaches. In this study, we compared 4,219Pstexpression sequence tags (ESTs) to the genomic sequence ofP. graminisf. sp.tritici(Pgt), the wheat stem rust fungus, using BLAST searches. The percentages of homologous genes varied greatly among differentPstlibraries with 54.51%, 51.21%, and 13.61% for the urediniospore, germinated urediniospore, and haustorial libraries, respectively, with an average of 33.92%. The 1,432Pstgenes with significant homology withPgtsequences were grouped into physical groups corresponding to 237Pgtsupercontigs. The physical relationship was demonstrated by 12 pairs (57%), out of 21 selectedPstgene pairs, through PCR screening of aPstBAC library. The results indicate that thePgtgenome sequence is useful in constructingPstphysical maps.

Funder

U.S. Department of Agriculture

Publisher

Hindawi Limited

Subject

Genetics,Molecular Biology,Biotechnology

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