Uncommon Trimethoxylated Flavonol Obtained fromRubus rosaefoliusLeaves and Its Antiproliferative Activity

Abstract

This study shows the evaluation the antiproliferative effect of the extract, fractions, and uncommon compounds isolated fromR. rosaefoliusleaves. The compounds were identified by conventional spectroscopic methods such as NMR-H1and C13and identified as 5,7-dihydroxy-6,8,4′-trimethoxyflavonol (1), 5-hydroxy-3,6,7,8,4′-pentamethoxyflavone (2), and tormentic acid (3). Both hexane and dichloromethane fractions showed selectivity for multidrug-resistant ovary cancer cell line (NCI-ADR/RES) with total growth inhibition values of 11.1 and 12.6 μg/ml, respectively. Compound1also showed selective activity against the same cell line (18.8 μg/ml); however, it was especially effective against glioma cells (2.8 μg/ml), suggesting that this compound may be involved with thein vitroantiproliferative action.