hsa_circ_0084811 Regulates Cell Proliferation and Apoptosis in Retinoblastoma through miR-18a-5p/miR-18b-5p/E2F5 Axis

Author:

Jiang Guangwei1,Qu Mingxuan2,Kong Lili3,Song Xiaoming3,Jiang Shanhao1ORCID

Affiliation:

1. Department of Ophthalmology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, China

2. Hemodialysis Room, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, China

3. Endocrinology & Metabolic Diseases, Yantai Affiliated Hospital of Binzhou Medical University, Yantai City, Shandong Province 264100, China

Abstract

Background. Retinoblastoma (RB) is the commonest primary intraocular malignancy during childhood. Circular RNAs (circRNAs) act as regulators in RB development, and hsa_circ_E2F5 (circ_0084811 in this study) was found to be highly expressed in RB cells, so we wanted to identify its detailed molecular mechanism. Methods. The expression level of circ_0084811 in RB cells was tested by RT-qPCR and its effects on RB cells were evaluated through functional assays. The regulatory mechanism that circ_0084811 may exert in RB progression was testified through mechanism experiments. Results. High circ_0084811 expression in RB cells facilitated cell proliferation but inhibited cell apoptosis. The enrichment of acetylation of histone 3 lysine 27 (H3K27ac) in circ_0084811 promoter induced circ_0084811 upregulation. Moreover, circ_0084811 regulated E2F transcription factor 5 (E2F5) expression via sponging microRNA-18a-5p (miR-18a-5p) and microRNA-18b-5p (miR-18b-5p). Conclusion. circ_0084811 modulated RB progression via the miR-18a-5p/miR-18b-5p/E2F5 axis.

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference37 articles.

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