The Link between ANRIL Gene RS4977574 Polymorphism and Common Atherosclerosis Cardiovascular Complications: A Hospital-Based Case-Control Study in Ukrainian Population

Abstract

The Aim. To test the possible association between ANRIL rs4977574 polymorphism and the development of atherosclerosis and cardiovascular complications (acute coronary syndrome (ACS) and large artery stroke (LAS)) in Ukrainian population. Materials and Methods. 195 patients with ACS, 200 patients with LAS, and 234 control subjects were enrolled in this case-control study. Real-time PCR was used for ANRIL rs4977574 genotyping. SPSS software package (version 17.0, IBM, USA) was used for data analysis. Results. A significant association between rs4977574 polymorphism and the risk of atherosclerosis and cardiovascular complications was found under the recessive model regardless of adjustment for nongenetic risk factors ( $\text{OR}=1.551$ ; $p=0.025$ ). Moreover, the link between rs4977574 locus and serum levels of total cholesterol ( $p=0.021$ ) and LDL ( $p=0.022$ ) was detected. A separate analysis in subgroups demonstrated the association of rs4977574 polymorphism with increased risk of ACS under the recessive model ( $\text{OR}=1.501$ ; $p=0.048$ ). No relation between rs4977574 site and LAS development was revealed ( $p>0.05$ ). Conclusion. Obtained data suggested that ANRIL rs4977574-GG genotype can be a possible genetic marker for the development of atherosclerosis and cardiovascular complications in Ukrainian population.