Effect of Low-Frequency Vibration on Muscle Response under Different Neurointact Conditions

Abstract

Stretch reflex is an important factor that influences the biomechanical response of the human body under whole-body vibration. However, there is a lack of quantitative evaluation at lower frequencies. Thus, the aim of this study was to investigate the effects of vibration on the stretch reflex and, in particular, to explore the quantitative relationship between dynamic muscle responses and low-frequency vibrations. The gastrocnemius muscle of 45 Sprague-Dawley rats was dissected. Sinusoidal vibrations of five discrete frequencies (2~16 Hz) with peak-to-peak amplitudes of 1 mm were applied to the gastrocnemius muscles with 2 mm or 3 mm prelengthening. Variables including dynamic muscle force, vibration acceleration, and displacement were recorded in two conditions, with and without the stretch reflex. Results showed that the dynamic muscle forces decreased by 20% on average for the 2 mm prelengthening group after the stretch reflex was blocked and by 24% for the 3 mm prelengthening group. Statistical analysis indicated that the amplitude of dynamic muscle force in the “with stretch reflex” condition was significantly larger than that in the “without stretch reflex” condition (p<0.001). The tension-length curve was found to be a nonlinear hysteresis loop that changed with frequency. The phase difference between the dynamic muscle force and the length change was affected significantly by vibration frequency (p<0.01), and the minimum frequency was 4–8 Hz. Experimental results of this study could benefit musculoskeletal model by providing a theoretical support to build a stretch reflex model for low-frequency vibration.