Effect of Phytofabricated Silver Oxide Nanoparticles on Wound Pathogens

Abstract

Infection control is a challenging task in the treatment of wounds and a rise in antimicrobial resistance wound pathogens which is a barrier for the wound regeneration rapidly. Therefore, there is an urgent requirement of novel antimicrobial agents to target the wound pathogens and their biofilms. Silver nanoparticles (AgNPs) are the predominant antimicrobial agent for wound treatment due to their broad spectrum antimicrobial potential against various wound pathogens. Silver nanoparticles (AgNPs) were prepared from aqueous Adenanthera pavonina seed extract. This extract plays as a reducing agent for reduction of silver nitrate to silver oxide nanoparticles. An absorption peak at 428 nm with characteristic feature of surface plasmon resonance was observed. The average size of AgNPs was found to be ~200 nm and cube in shape of AgNPs. FTIR data revealed the presence of phenolic groups that were responsible for reduction and stabilization of silver nanoparticles. Face centered cubic (FCC) crystalline structure was revealed from XRD relating to silver oxide (AgO) with good antibacterial potential. The antioxidant activity of extract increases when the concentration increases, and it can be used in the inhibition of free radicals at wound site. Antibacterial activities showed effective inhibition against various pathogens such as E.coli, Salmonella typhi, Sphingomonas, and Bacillus. Given this correspondence, the green synthesized AgNPs would be a potential antimicrobial agent for various wound treatments.