Exploring Antiurolithic Effects of Gokshuradi Polyherbal Ayurvedic Formulation in Ethylene-Glycol-Induced Urolithic Rats

Abstract

Gokshuradi Yog (GY)is a polyherbal ayurvedic formulation used traditionally for several decades in India for the treatment of urolithiasis. The aim of the present study was to determine the underlying mechanism ofGYaction in the management of calcium oxalate urolithiasis. The effect ofGokshuradi polyherbal aqueous extracts(GPAEs) was studied on various biochemical parameters involved in calcium oxalate formation by employingin vitroandin vivomethods.GPAEexhibited significant antioxidant activity against 1, 1-diphenyl-2-picrylhydrazyl free radical and inhibited lipid peroxidation in thein vitroexperiments. The rat model of urolithiasis induced by 0.75% ethylene glycol (EG) and 1% ammonium chloride (AC) in water caused polyuria, weight loss, impairment of renal function, and oxidative stress and decreased antioxidant enzyme activities in untreated control groups. However,GPAE-(25, 50, and 100 mg/kg) treated groups caused diuresis accompanied by a saluretic effect and revealed significant increase in antioxidant enzyme activities along with decreased oxalate synthesizing biochemical parameters at higher doses. This study revealed the antiurolithic effect ofGPAEmediated possibly through inhibiting biochemical parameters involved in calcium oxalate formation, along with its diuretic and antioxidant effects, hence supporting its use in the treatment of calcium oxalate urolithiasis.