Affiliation:
1. Department of Surgery, Clinical Hospital Center, Medical Faculty, University of Rijeka, 51000 Rijeka, Croatia
2. Department of Physiology and Immunology, Medical Faculty, University of Rijeka, 51000 Rijeka, Croatia
Abstract
Perforin-(P-) related characteristics of cytotoxic T lymphocytes and natural killer cells were investigated in peripheral blood of patients subjected to open (OC;n=23) or laparoscopic cholecystectomy (LC;n=21) and healthy controls (n=20). Blood samples were obtained preoperatively and 24 hours after the surgeries, and the data were correlated with the intensity of cholestasis and concomitant inflammation, determined by functional hepatic tests. Postoperative differences were found to be minimal: OC decreased only the percentage ofCD56+cells, while LC decreased the fraction ofCD8+P+cells and augmented the mean fluorescence intensity of P in CD56 cells. Patients elected for OC had, however, higher preoperative numbers of totalP+,CD3+P+, andCD4+P+cells than patients elected for LC and healthy controls, while both groups of patients, preoperatively, had lower fraction ofCD16+P+andCD56+P+cells. These changes were in high correlation with blood concentrations of CRP, AP, and ALT, emphasizing the link between the preoperative cholestasis and inflammation and P-dependent cytotoxic mechanisms.
Funder
Croatian Ministry of Science
Cited by
2 articles.
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