Simultaneous Determination of Lactulose and Lactose in Conserved Milk by HPLC-RID-Reference-Cited by-同舟云学术

Simultaneous Determination of Lactulose and Lactose in Conserved Milk by HPLC-RID

Published:2015 Issue: Volume:2015 Page:1-6
ISSN:2090-9063
Container-title:Journal of Chemistry
language:en
Short-container-title:Journal of Chemistry

Author:

Silveira Michelle Fernandes¹,Masson Lourdes Maria Pessôa¹,Martins José Francisco Pereira²,Álvares Thiago da Silveira³,Paschoalin Vânia Margaret Flosi³,Lázaro de la Torre César⁴⁵,Conte-Junior Carlos Adam³⁵

Affiliation:

1. Federal Institute of Rio de Janeiro, No. 88, Pereira de Almeida Street, 20260-100 Praça da Bandeira, RJ, Brazil

2. Department of Food Technology, Federal Rural University of Rio de Janeiro, 23890-000 Seropédica, RJ, Brazil

3. Institute of Nutrition, Federal University of Rio de Janeiro, Macaé Campus, Imburo Road, Ajuda, 27979-000 Macaé, RJ, Brazil

4. Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Major National University of San Marcos, Circunvalación Avenue, San Borja, Lima 41, Peru

5. Department of Food Technology, Fluminense Federal University, No. 64, 24230-340 Niteroi, RJ, Brazil

Abstract

Heat treatment is applied to dairy products to ensure microbiological quality and increase the shelf life. However, a suitable control of this process is necessary to guarantee nutritional and sensory quality. The aim of this study is to adapt the high performance liquid chromatography (HPLC) method for determination of lactulose and lactose content in commercial samples of UHT and sweetened condensed milk. The HPLC method used showed a good resolution of the analytes evaluated. The analyzed UHT milk samples presented levels for lactulose in accordance with the limit recommended by the International Dairy Federation. There was no significant variation in lactulose concentration for sweetened condensed milk samples. However, one sweetened condensed milk sample showed lactose level lower than the established values (10–12%).

Funder

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Publisher

Hindawi Limited

Subject

General Chemistry

Link

http://downloads.hindawi.com/journals/jchem/2015/185967.pdf

Reference40 articles.

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